Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 12, Pages 4499-4507Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.01.065
Keywords
Microalgae; Nitrogen limitation; Lipid biosynthesis; Fourier transform infrared spectroscopy; Biofuel
Funding
- Leverhulme Trust [F/00 120/AO, F/00 120/BG]
- BBSRC
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In this study Fourier transform infrared micro-spectroscopy (FTIR) was used to determine lipid and carbohydrate content over time in the freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus subspicatus grown in batch culture in limiting concentrations of nitrogen (N). Both algae exhibited restricted cell division and increased cell size following N-limitation. FTIR spectra of cells in N-limited media showed increasing lipid:amide 1 and carbohydrate:amide 1 ratios over time. The use of lipid- and starch-staining dyes confirmed that the observed ratio changes were due to increased lipid and carbohydrate synthesis. These results demonstrate rapid metabolic responses of C. reinhardtii and S. subspicatus to changing nutrient availability, and indicate the efficiency of FIR as a reliable method for high-throughput determination of lipid induction. (C) 2010 Elsevier Ltd. All rights reserved.
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