Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 6, Pages 2049-2052Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.10.078
Keywords
Enzymolysis pretreatment; Bio-hydrogen; Dark-fermentation; Photo-fermentation
Funding
- National Basic Research Program of China [2003CB214500, 2009CB220000]
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Enhanced bio-hydrogen production from pretreated corncob by integrating dark-fermentation with photo-fermentation process was investigated in this study. In the first step, the maximum bio-hydrogen yield and rate from corncob by dark-fermentation was 120.3 +/- 5.2 mL H-2/g-corncob and 150 mL H-2/(L h), respectively. In the second step, a hydrogen yield of 713.6 +/- 44.1 mL H-2/g-COD was obtained from digesting the effluent of dark-fermentation by photosynthetic bacteria. Meanwhile, COD removal efficiency achieved 90%. Bio-hydrogen production in the first step was mainly attributed to the bioconversion of the reducing sugars and oligosaccharides in the hydrolyzate of corncob. Bio-hydrogen production in the second step was due to the biodegradation of acetic acid, butyric acid, butyl alcohol and ethanol in the effluent of dark-fermentation. (C) 2009 Elsevier Ltd. All rights reserved.
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