Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 24, Pages 9631-9637Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.07.057
Keywords
Compost; Bacterial community; 16S rRNA; DGGE
Funding
- National Agriculture and Food Research Organization (NARO)
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The bacterial communities in the core, bottom, top, middle-surface, and lower-surface full-scale passively aerated cattle manure compost was investigated using DGGE of PCR-amplified 16S rRNA sequences. Some Bacillus species and strictly anaerobic thermophilic Clostridium species were dominant only in the core and bottom zones. In contrast, bands belonging to mesophilic bacteria such as Bacteroidetes, Clostoridia, alpha and gamma-proteobacteria were detected in surface zones, even in the initial thermophilic stage of the process. Our results clearly show the spatial distribution of the microbial community within full-scale composting piles, which indicates N or C conversion by zone-specific bacterial communities were occurring in each zone of the pile. (C) 2010 Elsevier Ltd. All rights reserved.
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