Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 6, Pages 1961-1967Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.10.052
Keywords
2,3-Butanediol; Serratia marcescens H30; Respiratory quotient; Medium optimization; Fed-batch
Funding
- Chinese National Program for High Technology and Development (863 Program) [2006AA02Z243]
- Shanghai Leading Academic Discipline [13505]
- State key laboratory special fund [2060204]
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The production of 2,3-butanediol (2,3-BD) by Serratia marcescens H30 from sucrose was studied. Medium composition for 2,3-BD production by S. marcescens H30 was optimized in shake flask fermentations using Plackett-Burman design (PB) and response surface methodology (RSM). Results indicated that yeast extract and sodium acetate had significant effects on the 2,3-BD production. And their optimal concentrations were determined by RSM. The optimal medium was used to perform fermentation experiments by S. marcescens H30 in a 3.71 bioreactor. Several feeding strategies including interim feeding, exponential feeding and constant residual sucrose concentration feeding were compared for improving 2,3-BD production. Ultimately, a suitable control strategy which combined the respiratory quotient (RQ) control with the constant residual sucrose concentration fed-batch was developed. Using this strategy, the maximum 2,3-BD concentration of 139.92 g/l with the diol (AC + BD) productivity of 3.49 g/l h and the yield of 94.67% was obtained. (C) 2009 Elsevier Ltd. All rights reserved.
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