Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 21, Pages 8376-8382Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.06.045
Keywords
Acidophilic alpha-galactosidase; Bispora sp MEY-1; Synergistic enzyme action
Funding
- Key Program of Transgenic Plant Breeding [2008ZX08003-002]
- National High Technology Research and Development Program of China (863 program) [2007AA100601]
- Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-42-G2-05]
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An alpha-galactosidase gene (AgalB) was cloned from the acidophilic fungus Bispora sp. MEY-1 and expressed in Pichia pastoris. The deduced amino acid sequence showed highest identity (35%) to the alpha-galactosidase from Penicillium simplicissimum, belonging to the glycosyl hydrolase family 27. The purified recombinant alpha-galactosidase (r-AgalB) exhibited optimal activity at pH 3.5 and 55 degrees C, was stable at pH 2.2-8.0, and showed higher hydrolytic activity towards galactomannan polysaccharides (guar gum and locust bean gum) than toward small galacto-oligosaccharides (melibiose, raffinose and stachyose). A synergistic (3-fold) increase in guar gum hydrolysis was observed when beta-mannanase Man5A from Bispora sp. MEY-1 and r-AgalB were combined. Further, an increase in the reaction time from 5 h to 12 h or increase of the temperature from 37 degrees C to 55 degrees C enhanced guar gum degradation by the enzyme combination. These properties make r-AgalB a good candidate for extensive application in the pulp/paper, food, and feed industries. (C) 2010 Elsevier Ltd. All rights reserved.
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