Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 14, Pages 5523-5527Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.02.021
Keywords
Kitasatospora sp MY 5-36; Immobilized cells; Poly-epsilon-lysine; Repeated fed-batch; Loofah sponge
Funding
- National Basic Research Program of China [2007CB714304]
- National High Technology Research and Development Program of China [2009AA033004]
- Jiangsu Key Technology Research and Development [BE2009363]
- Jiangsu Postdoctoral Sustentation Foundation [0901011C]
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The production of epsilon-PL by Kitasatospora sp. MY 5-36 through entrapment or adsorption on bagasse, synthetic sponge, macroporous silica gel, and loofah sponge was investigated in shake flask cultures, and immobilization on loofah sponge gave the highest epsilon-PL production. Repeated fed-batch cultures for epsilon-PL production were also carried out in a stirred bioreactor and final epsilon-PL concentrations and productivity of 34.11 g L-1 and 9.34 g L-1 d(-1), respectively were achieved by cells immobilize in loofah sponge. These values exceeded those for cultures with free cells (22.53 g L-1 and 3.30 g L-1 d(-1)). The immobilized cells were reused five times over a period of 526 h. These results suggest that the immobilization approach is promising for industrial applications. (C) 2010 Elsevier Ltd. All rights reserved.
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