Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 22, Pages 8902-8906Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.06.070
Keywords
Propionibacterium acidipropionici; Propionic acid; Glycerol; Scale-up; Optimization
Funding
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0532]
- National Science Fund for Distinguished Young Scholars of China [20625619]
- Fundamental Research Funds for the Central Universities [JUSRP30901]
- 973 Project [2007CB714306, 2010CB535014]
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Propionic acid production by Propionibacterium acidipropionici with glycerol as sole carbon source was studied in 7-L batch and 10 m(3) bioreactor cultures. In batch cultures, propionic acid production increased and specific cell growth rate decreased with increasing glycerol concentrations. Maximum propionic acid production and productivity reached 44.62 +/- 1.12 g/L and 0.20 +/- 0.0075 g L-1 hat 220 h, respectively, when glycerol was fed at a constant rate of 0.01 L/h from 72 to 120 h with an initial glycerol concentration of 30 g/L In the 10 m(3) bioreactor, maximum propionic acid production reached 47.28 +/- 0.12 g/L at 240 h. This glycerol feeding approach may be useful for propionic acid production on an industrial scale. (C) 2010 Elsevier Ltd. All rights reserved.
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