Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 12, Pages 4354-4361Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.01.094
Keywords
Bioprecipitation; Sulfate reducing bacteria; Heavy metal; Polyvinyl alcohol (PVA); Central composite design
Funding
- National Science Council, Taiwan, Republic of China [NSC-95-2211-E-264-017]
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The effect of a sulfate reducing bacteria immobilized in polyvinyl alcohol (PVA) on simultaneous sulfate reduction and copper removal was investigated. Batch experiments were designed using central composite design (CCD) with two parameters, i.e. the copper concentration (10-100 mg/L), and the quantity of immobilized SRB in culture solution (19-235 mg of VSS/L). Response surface methodology (RSM) was used to model the experimental data, and to identify optimal conditions for the maximum sulfate reduction and copper removal. Under optimum condition, i.e. similar to 138.5 mg VSS/L of sulfate reducing bacteria immobilized in PVA, and similar to 51.5 mg/L of copper, the maximum sulfate reduction rate was 1.57 d(-1) as based on the first-order kinetic equation. The data demonstrate that immobilizing sulfate reducing bacteria in PVA can enhance copper removal and the resistance of the bacteria towards copper toxicity. (C) 2010 Elsevier Ltd. All rights reserved.
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