Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 22, Pages 8742-8749Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.06.055
Keywords
Agaricus arvensis; Cellulase; Poplar wood; RSM optimization; Saccharification
Funding
- Konkuk University
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Agaricus arvensis, a newly isolated basidiomycetous fungus, was found to secrete efficient cellulases. The strain produced the highest endoglucanase (EG), cellobiohydrolase (CBH) and beta-glucosidase (BGL) activities of 0.3, 3.2 and 8 U/mg-protein, respectively, with rice straw as the carbon source. Saccharification of the woody biomass with A. arvensis cellulase as the enzyme source released a high level of fermentable sugars. Enzymatic hydrolysis of the poplar biomass was optimized using the response surface methodology in order to study the influence of the variables (pH, temperature, cellulases concentration and substrate concentration). The enzyme and substrate concentrations were identified as the limiting factors for the saccharification of poplar wood biomass. A total reducing sugar level of 29 g/L (293 mg/g-substrate) was obtained at an enzyme concentration of 65 FPU/g-substrate after optimization of the hydrolysis parameters. The model validation showed a good agreement between the experimental results and the predicted responses. A. arvensis could be a good candidate for the production of reducing sugars from a cellulosic biomass. (C) 2010 Elsevier Ltd. All rights reserved.
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