Journal
BIORESOURCE TECHNOLOGY
Volume 101, Issue 11, Pages 4151-4156Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.01.043
Keywords
L-Phenylalanine; Feedback-resistant; pheA(fbr) gene; aroF(wt) gene; Biosynthesis
Funding
- National Science Fund for Distinguished Young Scholars [20625619]
- National Natural Science Foundation of China [20836003]
- Key Project of Chinese National Programs for Fundamental Research and Development (973 program) [2007CB71403]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0532]
- Jiangnan University [JUSRP10917]
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A beta-2-thienylalanine-resistant E. coli K12 mutant carrying a Thr326Pro mutation in the regulation (R) domain of A (pheA(fbr)) was obtained by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis. In the presence of 200 mM L-phenylalanine (L-Phe), a recombinant E. coli WSH-Z06 (pAP-B03) carrying pheA(fbr) as well as wild-type aroF (aroF(wt)) exhibited more than 70% of the chorismate mutase-prephenate dehydratase (CM-PDT) activity as observed in the absence of this amino acid. The L-Phe titer of WSH-Z06 (pAP-B03) reached 35.38 g/L in a 3-L fermentor, which was 2.81-fold higher than that of the original strain E. coli WSH-Z06. Furthermore, the L-Phe yield on glucose of WSH-Z06 (pAP-B03) (0.26 mol/mol) was twice that of E. coli WSH-Z06. This recombinant E. coli WSH-Z06 (pAP-B03) is a potential strain for over-production of L-Phe. (C) 2010 Published by Elsevier Ltd.
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