Journal
TOXICOLOGICAL RESEARCH
Volume 34, Issue 1, Pages 13-21Publisher
KOREAN SOC TOXICOLOGY
DOI: 10.5487/TR.2018.34.1.013
Keywords
Vitis coignetiae pulliat; Anthocyanins; 3T3-L1 preadipocytes; Adipogenesis; AMPK
Categories
Funding
- Blue-Bio Industry Regional Innovation Center at Dongeui University as a RIC program under Ministry of Trade, Industry Energy [RIC08-06-07]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Korea government [2015R1A2A2A01004633]
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Anthocyanins are naturally occurring water-soluble polyphenolic pigments in plants that have been shown to protect against cardiovascular diseases, and certain cancers, as well as other chronic human disorders. However, the anti-obesity effects of anthocyanins are not fully understood. In this study, we investigated the effects of anthocyanins isolated from the fruit of Vitis coignetiae Pulliat on the adipogenesis of 3T3-L1 preadipocytes. Our data indicated that anthocyanins attenuated the terminal differentiation of 3T3-L1 preadipocytes, as confirmed by a decrease in the number of lipid droplets, lipid content, and triglyceride production. During this process, anthocyanins effectively enhanced the activation of the AMP-activated protein kinase (AMPK); however, this phenomenon was inhibited by the co-treatment of compound C, an inhibitor of AMPK. Anthocyanins also inhibited the expression of adipogenic transcription factors, including peroxisome proliferator-activated receptor-gamma, CCAAT/ enhancer-binding protein a and b, and sterol regulatory element-binding protein-1c. In addition, anthocyanins were found to potently inhibit the expression of adipocyte-specific genes, including adipocyte fatty acid-binding protein, leptin, and fatty acid synthase. These results indicate that anthocyanins have potent anti-obesity effects due to the inhibition of adipocyte differentiation and adipogenesis, and thus may have applications as a potential source for an anti-obesity functional food agent.
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