4.8 Article

Development of the cellulolytic fungus Trichoderma reesei strain with enhanced beta-glucosidase and filter paper activity using strong artifical cellobiohydrolase 1 promoter

Journal

BIORESOURCE TECHNOLOGY
Volume 101, Issue 24, Pages 9815-9818

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2010.07.078

Keywords

Trichoderma reesei; beta-Clucosidase 1; Cellulase; Modified four-copy cbh1 promoter

Funding

  1. National Natural Science Foundation of China [30670029, 30800024]
  2. National High Technology Research and Development Program of China [2007AA05Z455]

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To improve the beta-glucosidase yield and total cellulase activity of Trichoderma reesei, extracellular beta-glucosidase (BGLI) was overexpressed under the control of the modified four-copy cbh1 promoter. Three transformants B2, B12 and B15 with successful integration of the bgl1 gene expression cassette were obtained, which exhibited 3.7-, 2.0- and 1.8-fold increase in beta-glucosidase activity than the parental strain RUT C30. respectively. The filter paper activities of the productive transformants 612 and 615 were improved by up to 130% and 55%, respectively. Saccharification of corncob residue with the crude enzyme dosages showed that the reducing sugar yields of B12 (5.59 mg/ml) and 615 (4.80 mg/ml) were 29% and 11% higher than that of RUT C30 (4.34 mg/ml). respectively. The present results proved that the modified four-copy cbh1 promoter was a useful tool for improving the cellulase activity of T. reesei, and the engineering strains developed in this study could be potentially used as promising cell factories for beta-glucosidase or cellulase production. (c) 2010 Elsevier Ltd. All rights reserved.

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