Journal
BIORESOURCE TECHNOLOGY
Volume 100, Issue 17, Pages 3921-3926Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.03.019
Keywords
Semi-continuous cultivation; Nitrogen limitation; Bio-fuel; Lipid; Chlorella sp.
Funding
- National Science Council of Taiwan [96-2221-E-006045]
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The microalgae. Chlorella sp., were cultivated in various culture modes to assess biomass and lipid productivity in this study. In the batch mode, the biomass concentrations and lipid content of Chlorella sp. cultivated in a medium containing 0.025-0.200 g L-1 urea were 0.464-2.027 g L-1 and 0.661-0.326 g g(-1), respectively. The maximum lipid productivity of 0.124 g d(-1) L-1 occurred in a medium containing 0.100 g L-1 urea. In the fed-batch cultivation, the highest lipid content was obtained by feeding 0.025 g L-1 of urea during the stationary phase, but the lipid productivity was not significantly increased. However, a semi-continuous process was carried out by harvesting the culture and renewing urea at 0.025 g L-1 each time when the cultivation achieved the early stationary phase. The maximum lipid productivity of 0.139 g d(-1) L-1 in the semi-continuous culture was highest in comparison with those in the batch and fed-batch cultivations. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.
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