Journal
BIORESOURCE TECHNOLOGY
Volume 100, Issue 21, Pages 5230-5236Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.05.038
Keywords
Trichoderma; Endoxylanase; Xylooligosaccharide; Xylan; Characterization
Funding
- Hi-Tech Research and Development program of China [2007AA091504, 2007AA091903]
- COMRA Program [DYXM-115-02-2-6]
- Specialized Research Fund for the Doctoral Program of Higher Education [20060422053]
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Trichoderma sp. K9301 secreting endoxylanases with an activity of 2836 U/g (dry weight) was screened for XOs production. Two acidic beta-endoxylanases EX1 (30.1 kDa) and EX2 (20.1 kDa) were purified from crude extract of the strain K9301 in solid fermentation. Action modes of EX1 and EX2 towards XOs showed similar hydrolysis characters to endoxylanases belonging to glycosyl hydrolase family 10 and 11, respectively. EX1 exhibited better affinity but lower hydrolytic efficiency than EX2 to xylans from beechwood, birchwood, and oat-spelt. They had synergistic action on xylan hydrolysis. The optimum condition to prepare XOs from corncobs was obtained as 10 mg/ml corncob xylan incubated with 10 U/mg crude enzymes at 50 degrees C for 3 h. The yield of XOs reached 43.3%, and only a little amount of xylose (3.1%) was simultaneously produced, suggesting the good potential of strain K9301 in XOs production. (C) 2009 Elsevier Ltd. All rights reserved.
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