Journal
BIORESOURCE TECHNOLOGY
Volume 100, Issue 14, Pages 3644-3649Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.02.037
Keywords
Medicinal mushroom; Tuber melanosporum; Fed-batch fermentation; Tuber polysaccharides
Funding
- National Natural Science Foundation of China (NSFC) [20706012]
- National High Technology Research and Development Key Program of China [2007AA021506]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars (Ministry of Personnel), Hubei Provincial Innovative Research Team in University [T200608]
- Hubei Provincial Natural Science Foundation for Innovative Research Team [2008CDA002]
- Hubei Provincial International Cooperation Foundation for Scientific Research [2007CA012]
- Scientific Research Key Foundation from Hubei University of Technology [306.18002]
- Open Project Program of the State Key Laboratory of Bioreactor Engineering (ECUST)
- Hubei Provincial Department of Education, China
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For the first time, a fed-batch fermentation process of Tuber melanosporum was developed for the efficient production of bioactive mycelia and Tuber polysaccharides. Each 1.67 g/L of peptone and 8.33 g/L of yeast extract were added on day 3, 6, and 9, respectively, and sucrose was fed to maintain its concentration around 35-5 g/L when its residual level decreased to 10-5 g/L. Then, the maximal biomass, the production of extracellular polysaccharides (EPS) and intracellular polysaccharides (IPS) reached 53.72 +/- 2.57 g DW/L, 7.09 +/- 0.62 and 4.43 +/- 0.21 g/L, respectively. Compared with the batch culture conducted in the enriched medium, the biomass, the production of EPS and IPS were enhanced by 55.8%, 222.3% and 103.2%, respectively. Not only the cell density but also the production of EPS and IPS were the highest ever reported in truffle fermentation, and the biomass was also the highest as ever reported in mushroom fermentation. (C) 2009 Elsevier Ltd. All rights reserved.
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