Journal
BIORESOURCE TECHNOLOGY
Volume 100, Issue 23, Pages 5574-5580Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2009.06.060
Keywords
Low cost substrate; Response surface analysis; Cashew apple juice; Oligosaccharides
Funding
- CNPq
- CAPES
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The use of agriculture substrates in industrial biotechnological processes has been increasing because of their low cost. In this work, the use of clarified cashew apple juice was investigated as substrate for enzyme synthesis of prebiotic oligosaccharide. The results showed that cashew apple juice is a good source of reducing sugars and can be used as substrate for the production of dextransucrase by Leuconostoc citreum B-742 for the synthesis of oligosaccharides using the crude enzyme. Optimal oligosaccharide yield (approximately 80%) was obtained for sucrose concentrations lower than 60 g/L and reducing sugar concentrations higher than 100 g/L. (C) 2009 Elsevier Ltd. All rights reserved.
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