Journal
BIORESOURCE TECHNOLOGY
Volume 99, Issue 17, Pages 8524-8527Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2008.03.007
Keywords
Volvariella volvacea; Agrobacterium-mediated transformation; antifreeze protein gene; cold resistance
Funding
- National Natural Science Foundation of China [30371000]
- Hi-Tech Research and Development Program of China (863 Program) [2006AA10Z301]
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Agrobacterium tumefaciens-mediated transformation (ATMT) was successfully applied to the edible straw mushroom, Volvariella volvacea. Mycelium pellets were transformed to cold stress resistance using the afp gene as both a selective marker and a reporter gene, under the control of a heterologous Lentinula edodes gpd promoter. The efficiency of transformation is over 100 times higher than that previously reported in V. volvacea. Stable integration of the afp gene with 1-4 copy numbers was confirmed in all 10 randomly selected transgenic events by Southern blot analysis. The mitotic stability of the transformants was demonstrated after five successive transfers on PDA medium without selection pressure and the PCR analysis of basidiospores harvested from transformants. (c) 2008 Elsevier Ltd. All rights reserved.
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