Journal
BIORESOURCE TECHNOLOGY
Volume 99, Issue 14, Pages 6325-6331Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2007.12.012
Keywords
biohydrogen; hydrogen; fermentation; enzyme; sewage
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Hydrogen was successfully produced by fermenting primary sewage sludge which had been both heat treated and digested with a commercially available enzyme preparation. When either heat treatment or enzymatic digestion were not used, no hydrogen was produced during fermentation. Heat treated mesophilic anaerobic sludge was used as an inoculum rather than a pure microbial culture. Fermentation was conducted at pH levels ranging from of 4.5 to 7.0. When fermentation took place at pH 5.5 a peak hydrogen production rate of 3.75 ml min(-1) was observed. At this pH the hydrogen yield was 0.37 mol H-2 mol(-1) carbohydrate, equivalent to 18.14 L H-2 kg(-1) dry solids. (C) 2007 Elsevier Ltd. All rights reserved.
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