Journal
BIORESOURCE TECHNOLOGY
Volume 99, Issue 7, Pages 2242-2249Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2007.05.006
Keywords
alkaline protease; Bacillus; optimization; Plackett-Burman design; response surface methodology
Funding
- Korea Institute of Industrial Technology(KITECH) [2007-N-CT-HM-E-02-0000] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [과06B1213] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The proteolytic enzymes are the most important group of commercially produced enzymes. The production of alkaline protease was optimized using a newly isolated Bacillus sp. RKY3. The fermentation variables were selected in accordance with the Plackett-Burman design and were further optimized via response surface methodological approach. Four significant variables (corn starch, yeast extract, corn steep liquor, and inoculum size) were selected for the optimization studies. The statistical model was constructed via central composite design (CCD) using three screened variables (corn starch, corn steep liquor, and inoculum size). An overall 2.3-fold increase in protease production was achieved in the optimized medium as compared with the unoptimized basal medium. Enzyme activity increased significantly with optimized medium (939 u ml(-1)) when compared with unoptimized medium (417 u ml(-1)). (C) 2007 Elsevier Ltd. All rights reserved.
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