Journal
SYNTHETIC BIOLOGY
Volume 3, Issue 1, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/synbio/ysy012
Keywords
synthetic biology; high-throughput screening; cell-free systems; directed evolution
Funding
- Society-in-Science: Branco-Weiss Fellowship
- Human Frontier Science Program [RGY0080/2015]
- UC MEXUS-CONACYT doctoral fellowship
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The incorporation of cell-free transcription and translation systems into high-throughput screening applications enables the in situ and on-demand expression of peptides and proteins. Coupled with modern microfluidic technology, the cell-free methods allow the screening, directed evolution and selection of desired biomolecules in minimal volumes within a short timescale. Cell-free high-throughput screening applications are classified broadly into in vitro display and on-chip technologies. In this review, we outline the development of cell-free high-throughput screening methods. We further discuss operating principles and representative applications of each screening method. The cell-free high-throughput screening methods may be advanced by the future development of new cell-free systems, miniaturization approaches, and automation technologies.
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