Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 38, Issue 3, Pages 523-530Publisher
SPRINGER
DOI: 10.1007/s00449-014-1292-4
Keywords
Microalgae; Chlorella protothecoides; Brewery wastewater; Biodiesel production; Harvesting
Funding
- Advanced Biomass R&D Center (ABC) of the Global Frontier Project - Ministry of Science, ICT and Future Planning [ABC-20100029728]
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The use of wastewater has been investigated to overcome the economic challenge involved with a production of microalgae-based biodiesel. In this study, to achieve economical biodiesel production along with effective wastewater treatment at the same time, anaerobically treated brewery wastewater (ABWW) was utilized as a low-cost nutrient source, in the cultivation of Chlorella protothecoides. About 96 and 90 % of total nitrogen and phosphorus in ABWW were removed, respectively, while C. protothecoides was accumulating 1.88 g L-1 of biomass. The C. protothecoides grown in ABWW showed increases in cell size and cell aggregation, resulting in a near 80 % enhanced harvesting efficiency within 20 min, as compared with only 4 % in BG-11. In addition, the total fatty acid content of the C. protothecoides grown in ABWW increased by 1.84-fold (35.94 +/- A 1.54 % of its dry cell weight), relative to that of BG-11.
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