Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 37, Issue 3, Pages 505-511Publisher
SPRINGER
DOI: 10.1007/s00449-013-1015-2
Keywords
Mortierella alpina; Single-cell oils; Arachidonic acid; Three-stage fermentation
Funding
- National Science Foundation for Distinguished Young Scholars of China [21225626]
- National Basic Research Program of China [2011CBA00807]
- National Natural Science Foundation of China [21006049]
- National Key Technology Support Program of China [2011BAD23B03]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1066]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Program for New Century Excellent Talents in University from the Ministry of Education of China [NCET-09-0157]
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A three-stage fermentation strategy was designed for efficient arachidonic acid (ARA)-rich oil production by Mortierella alpina. The process at different stages by changing the components of medium was investigated. In the first stage, mycelia were inoculated in a nutrient-rich medium for rapid propagation. In the second stage, mycelia were collected and then cultivated in glucose solution to achieve high cellular lipid contents. In the third stage, mycelia were cultured in a glucose-absent medium to obtain rapid ARA accumulation. Using this fermentation strategy, high dry cell weight, lipid, and ARA concentration reached 41.6, 26.6, and 11.4 g/L, respectively. The results demonstrated that mycelia propagation, lipid biosynthesis, and ARA accumulation process can be significantly spatially separated, allowing further optimization to improve the efficiency of each stage. This was the first report of using a three-stage fermentation strategy for ARA-rich oil production, and it could be applied to other similar oleaginous microorganisms to obtain high related polyunsaturated fatty acids accumulation.
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