Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 36, Issue 10, Pages 1463-1474Publisher
SPRINGER
DOI: 10.1007/s00449-013-0885-7
Keywords
Polyhydroxybutyrate; High cell density production; Fed-batch cultivation; Fermentation scale-up; Sugarcane molasses; Bacillus megaterium
Funding
- National Innovation Agency (NIA), Ministry of Science and Technology, Thailand
- Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission [AM1026A-55]
- Integrated Innovation Academic Center: IIAC Chulalongkorn University Centenary Academic Development Project [CU56-AM05]
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This study demonstrated the improved polyhydroxybutyrate (PHB) production via high cell density cultivation of Bacillus megaterium BA-019 with balanced initial total sugar concentration and carbon to nitrogen (C/N) weight ratio. In the 10 L stirred fermentor operated at 30 A degrees C, pH 7.0, 600 rpm, and 1.0 vvm air, with the initial total sugar concentration of 60 g/L and urea at the C/N weight ratio of 10:1, 32.48 g/L cell biomass with the corresponding PHB weight content of 26.94 % and volumetric productivity of 0.73 g/L h were obtained from batch cultivation. Continuing cultivation by intermittent feeding of the sugarcane molasses along with urea at the C/N weight ratio of 12.5:1 gave much improved biomass and PHB production (90.71 g/L biomass with 45.84 % PHB content and 1.73 g/L h PHB productivity). Similar biomass and PHB yields were obtained in the 90 L stirred fermentor when using the impeller tip speed as the scale-up criterion.
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