Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 37, Issue 7, Pages 1411-1416Publisher
SPRINGER
DOI: 10.1007/s00449-013-1113-1
Keywords
Piceid-beta-D-glucosidase; Biotransformation; Resveratrol; Polydatin; Polygonum cuspidatum
Funding
- Agriculture Science Technology Achievement Transformation Fund [2012GB2B020506]
- Program for Liaoning Innovative Research Team in University [LT2012011]
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Resveratrol is now gaining much attention because of its pharmacological properties. Polygonum cuspidatum has the highest content of resveratrol in plants and is the best material offering resveratrol. However, the content of resveratrol in P. cuspidatum is much lower compared with its glycoside polydatin. In this study, enzymatic transformation of polydatin to resveratrol by piceid-beta-d-glucosidase from Aspergillus oryzae sp. 100 was investigated. The biotransformation conditions were optimized. Under the optimized conditions of 60 A degrees C, pH 5.0, substrate concentration of 40 g/L and piceid-beta-d-glucosidase activity of 5 U/mL, enzymatic transformation of polydatin from P. cuspidatum was successfully performed, during which 22.5 g/L of resveratrol was produced after reacting for 4 h, with the substrate conversion rate of 2 g/h/U of piceid-beta-d-glucosidase. A feasible and environment friendly process of enzymatic transformation of polydatin to resveratrol was developed, which provides a promising and competitive alternative for the production of resveratrol.
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