Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 35, Issue 8, Pages 1287-1295Publisher
SPRINGER
DOI: 10.1007/s00449-012-0716-2
Keywords
Kluyveromyces fragilis beta-galactosidase; Magnetic nanosized epoxy support; Covalent immobilization; Galacto-oligosaccharide
Funding
- Ministry of Science and Technology of People's Republic of China [2008AA10Z301]
- Tianjin Municipal Education Commission [060021]
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The novel magnetic nanobeads with epoxy groups on the surface were prepared from glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA) and hydroxyethyl methacrylate (HEMA) via emulsifier-free emulsion polymerisation, and they were characterized by scanning electron microscopy and vibrating sample magnetometer. The magnetic poly(GMA-EDGMA-HEMA) nanobeads were used as support for covalent immobilization of Kluyveromyces fragilis beta-galactosidase, the maximum amount enzyme attached onto the support was 145.6 mg/g with activity recovery of 72.6%. The loading capacity of this novel support for K. fragilis beta-galactosidase was improved 2.6-folds compared with Eupergit(A (R)) C (commercial epoxy support). The immobilized K. fragilis beta-galactosidase exhibited high catalytic activity for the reaction of galacto-oligosaccharide (GOS) synthesis, and a total of 2,240 g GOS were produced per gram of immobilized enzyme during consecutive batch reaction of 10 times. The immobilized biocatalyst retained 81.5% of its original activity after 10 reaction cycles.
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