Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 35, Issue 9, Pages 1467-1475Publisher
SPRINGER
DOI: 10.1007/s00449-012-0736-y
Keywords
Isobutanol; Engineered Saccharomyces cerevisiae; 2-Ketoisovalerate decarboxylase; Valine biosynthetic enzymes
Funding
- Advanced Biomass R&D Center (ABC) of Korea
- Ministry of Education, Science and Technology [2010-0029799]
- Marine Biotechnology Program
- Ministry of Land, Transport and Maritime Affairs of Korean Government
Ask authors/readers for more resources
Engineering of Saccharomyces cerevisiae to produce advanced biofuels such as isobutanol has received much attention because this yeast has a natural capacity to produce higher alcohols. In this study, construction of isobutanol production systems was attempted by overexpression of effective 2-keto acid decarboxylase (KDC) and combinatorial overexpression of valine biosynthetic enzymes in S. cerevisiae D452-2. Among the six putative KDC enzymes from various microorganisms, 2-ketoisovalerate decarboxylase (Kivd) from L. lactis subsp. lactis KACC 13877 was identified as the most suitable KDC for isobutanol production in the yeast. Isobutanol production by the engineered S. cerevisiae was assessed in micro-aerobic batch fermentations using glucose as a sole carbon source. 93 mg/L isobutanol was produced in the Kivd overexpressing strain, which corresponds to a fourfold improvement as compared with the control strain. Isobutanol production was further enhanced to 151 mg/L by additional overexpression of acetolactate synthase (Ilv2p), acetohydroxyacid reductoisomerase (Ilv5p), and dihydroxyacid dehydratase (Ilv3p) in the cytosol.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available