Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 36, Issue 9, Pages 1185-1190Publisher
SPRINGER
DOI: 10.1007/s00449-012-0845-7
Keywords
Escherichia coli; HydHG two-component system; zraP promoter; Zinc; Self-activation loop
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010-0012850]
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Characterizing the dynamics of HydHG-a two-component transcriptional regulatory network for exogenous zinc in E. coli-is essential in understanding the biology of these regulatory and signaling pathways. Here, we used a synthetic biology strategy to modify the dynamic characteristics of the HydHG network in two ways. First, a self-activation loop for HydHG network was created under the control of zraP promoter, after which the threshold Zn2+ concentration for the self-activated HydHG network significantly decreased from 200 to 10 mu M. Second, the self-activation loop was integrated into the E. coli genome allowing the threshold Zn2+ concentration to be elevated to 500 mu M. As the threshold Zn2+ concentration could be modified in both directions, the introduction of a self-activation loop and the entire genomic integration strategy may prove useful for the creation of a two-component bacterial biosensor with varying sensitivities.
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