Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 35, Issue 8, Pages 1355-1365Publisher
SPRINGER
DOI: 10.1007/s00449-012-0724-2
Keywords
Polyaniline; Polyacrylonitrile; Graft copolymerization; Immobilization; Horseradish peroxidase; Dye decolorization
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Horseradish peroxidase (HRP) was immobilized on the polyaniline (PANI) grafted polyacrylonitrile (PAN) films. The maximum HRP immobilization capacity of the PAN-g-PANI-3 film was 221 mu g/cm(2). The HRP-immobilized PAN-g-PANI-3 film retained 79 % of the activity of the same quantity free enzyme. The HRP-immobilized PAN-g-PANI-3 film was operated for the decolorization of two different benzidine-based dyes in the presence of hydrogen peroxide. The maximum decolorization grade was obtained at pH 6.0 for both dyes. The HRP-immobilized PAN-g-PANI-3 film was very effective for removal of Direct Blue-53 compared to Direct Black-38 from aqueous solutions. The immobilized HRP exhibited high resistance to proteolysis by trypsin compared to the free counterpart. Immobilized HRP preserved 83 % of its original activity even after 8 weeks of storage at 4 A degrees C, while the free enzyme lost its initial activity after 3 weeks of storage period.
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