Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 35, Issue 1-2, Pages 159-165Publisher
SPRINGER
DOI: 10.1007/s00449-011-0603-2
Keywords
Glycerol; Klebsiella pneumoniae; 1,3-Propanediol; By-product formation; Two-stage fermentation; Nutrient co-supplementation
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We generated a genetically engineered Klebsiella pneumoniae strain (AK-VOT) to eliminate by-product formation during production of 1,3-propanediol (1,3-PD) from glycerol. In the present study, the glycerol-metabolizing properties of the recombinant strain were examined during fermentation in a 5 L bioreactor. As expected, by-product formation was completely absent (except for acetate) when the AK-VOT strain fermented glycerol. However, 1,3-PD productivity was severely reduced owing to a delay in cell growth attributable to a low rate of glycerol consumption. This problem was solved by establishing a two-stage process separating cell growth from 1,3-PD production. In addition, nutrient co-supplementation, especially with starch, significantly increased 1,3-PD production from glycerol during fed-batch fermentation by AK-VOT in the absence of by-product formation.
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