Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 34, Issue 9, Pages 1143-1150Publisher
SPRINGER
DOI: 10.1007/s00449-011-0565-4
Keywords
Glucose biosensor; Multiwall carbon nanotubes; Titanium dioxide; Prussian blue; Functionalized gold nanoparticles
Funding
- National Natural Science Foundation of China [21075100]
- Ministry of Education of China [708073]
- Nature Science Foundation of Chongqing City [CSTC-2009BA1003]
- Specialized Research Fund for the Doctoral Program of Higher Education [20100182110015]
- State Key Laboratory of Electroanalytical Chemistry [SKIEAC 2010009]
- Southwest University
- High Technology Project Foundation of Southwest University, China [XSGX 02]
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In this paper, a new glucose biosensor was prepared. At first, Prussian blue (PB) was electrodeposited on a glassy carbon electrode (GCE) modified by titanium dioxide-multiwall carbon nanotubes-chitosan (TiO2-MWNTsCS) composite, and then gold nanoparticles functionalized by poly(diallyldimethylammonium chloride) (PDDA-Au) were adsorbed on the PB film. Finally, the negatively charged glucose oxidase (GOD) was self-assembled on to the positively charged PDDA-Au. The electrochemical performances of the modified electrodes had been studied by cyclic voltammetry (CV) and amperometric methods, respectively. In addition, the stepwise fabrication process of the as-prepared biosensor was characterized by scanning electron microscopy. PDDA-Au nanoparticles were characterized by ultraviolet-vis absorption spectroscopy and transmission electron microscopy. Under the optimal conditions, the as-prepared biosensor exhibited a good response performance to glucose with a linear range from 6 mu M to 1.2 mMwith a detection limit of 0.1 mu M glucose (S/N = 3). In addition, this work indicated that TiO2-MWNTs-CS composite and PDDA-Au nanoparticles held great potential for constructing biosensors.
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