Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 35, Issue 5, Pages 801-807Publisher
SPRINGER
DOI: 10.1007/s00449-011-0662-4
Keywords
Ethanol; Dilute pretreatment; Simultaneous saccharification and fermentation; Jatropha curcas
Funding
- Brazilian foundation CNPq
- Brazilian foundation CAPES
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Jatropha curcas has great potential as an oil crop for use in biodiesel applications, and the outer shell is rich in lignocellulose that may be converted to ethanol, giving rise to the concept of a biorefinery. In this study, two dilute pretreatments of 0.5% H2SO4 and 1.0% NaOH were performed on Jatropha shells with subsequent simultaneous saccharification and fermentation (SSF) of the pretreated water-insoluble solids (WIS) to evaluate the effect of inhibitors in the pretreatment slurry. A cellulase loading of 15 FPU/g WIS, complimented with an excess of cellobiase (19.25 U/g), was used for SSF of either the washed WIS or the original slurry to determine the effect of inhibitors. Ethanol and glucose were monitored during SSF of 20 g of pretreated biomass. The unwashed slurry showed to have a positive effect on SSF efficiency for the NaOH-pretreated biomass. Maximum efficiencies of glucan conversion to ethanol in the WIS were 40.43% and 41.03% for the H2SO4- and NaOH-pretreated biomasses, respectively.
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