Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 34, Issue 1, Pages 113-119Publisher
SPRINGER
DOI: 10.1007/s00449-010-0452-4
Keywords
Immobilization; Alginate lyase; Alginate oligosaccharide; Streptomyces sp ALG-5
Funding
- Korea Ministry of Knowledge Economy (MKE) [20093020090020]
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The marine alginate lyase from Streptomyces sp. ALG-5, which specifically degrades poly-G block of alginate, was functionally expressed as a His-tagged form with an Escherichia coli expression system. The recombinant alginate lyase expressed with pColdI at 15 A degrees C exhibited the highest alginate-degrading activity. The recombinant alginate lyase was efficiently immobilized onto two types of magnetic nanoparticles, superparamagnetic iron oxide nanoparticle, and hybrid magnetic silica nanoparticle, based on the affinity between His-tag and Ni2+ that displayed on the surfaces of nanoparticles. An alginate oligosaccharide mixture consisting of dimer and trimer was prepared by the immobilized alginate lyase. The immobilized enzymes were re-used repeatedly more than 10 times after magnetic separation.
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