Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 33, Issue 1, Pages 47-53Publisher
SPRINGER
DOI: 10.1007/s00449-009-0368-z
Keywords
Two-photon adsorbed photopolymerization; Glucose sensor; GOD (Glucose oxidase); Potassium ferrocyanide; Carbon nanotube; Three electrode; Micro-flow
Funding
- KOSEF (Korea) [R01-2007-000-20499-0]
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A novel glucose sensor was constructed, and its analytical potential examined. A chip-type three-electrode system for use in a flow-type electrochemical glucose sensor was fabricated using a UV lithography technique on a glass slide. An Ag/AgCl reference electrode was made by electroplating silver onto a Pt electrode and dipping in a saturated KCl solution for 30 min. In addition, a glucose-sensing electrode was fabricated using a two-photon adsorbed photopolymerization technique with a photo-reactive resin containing a glucose oxidase enzyme, ferrocene mediator, non-ionic surfactant, and carbon nanotubes. The cyclic voltammetry of the potassium ferrocyanide in the Pt sensor system showed a stable electrode condition. The response of the modified Pt sensor confirms the feasibility of using a two-photon adsorbed photopolymerization technique for the easy fabrication of functional biosensors.
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