Journal
BIOPOLYMERS
Volume 99, Issue 12, Pages 923-954Publisher
WILEY
DOI: 10.1002/bip.22347
Keywords
hydrogen exchange; formaldehyde probing; protein-nucleic acid interactions; DNA replication; DNA base analogue spectroscopy; single molecule DNA-protein interactions studies; two-dimensional fluorescence spectroscopy
Categories
Funding
- NIH [GM-15792, GM-29158]
- American Cancer Society
- NSF Chemistry of Life Processes Program [CHE-1105272]
- Office of Naval Research [N00014-11-0193]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1105272] Funding Source: National Science Foundation
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The coding sequences for genes, and much other regulatory information involved in genome expression, are located inside' the DNA duplex. Thus the macromolecular machines that read-out this information from the base sequence of the DNA must somehow access the DNA interior. Double-stranded (ds) DNA is a highly structured and cooperatively stabilized system at physiological temperatures, but is also only marginally stable and undergoes a cooperative melting phase transition at temperatures not far above physiological. Furthermore, due to its length and heterogeneous sequence, with AT-rich segments being less stable than GC-rich segments, the DNA genome melts' in a multistate fashion. Therefore the DNA genome must also manifest thermally driven structural (breathing) fluctuations at physiological temperatures that should reflect the heterogeneity of the dsDNA stability near the melting temperature. Thus many of the breathing fluctuations of dsDNA are likely also to be sequence dependent, and could well contain information that should be readable and useable by regulatory proteins and protein complexes in site-specific binding reactions involving dsDNA opening. Our laboratory has been involved in studying the breathing fluctuations of duplex DNA for about 50 years. In this Reflections article we present a relatively chronological overview of these studies, starting with the use of simple chemical probes (such as hydrogen exchange, formaldehyde, and simple DNA melting proteins) to examine the local stability of the dsDNA structure, and culminating in sophisticated spectroscopic approaches that can be used to monitor the breathing-dependent interactions of regulatory complexes with their duplex DNA targets in real time. (c) 2013 Wiley Periodicals, Inc. Biopolymers 99: 923-954, 2013.
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