Biological Activity of Laminin Peptide-Conjugated Alginate and Chitosan Matrices

Laminin active peptide conjugated chitosan mambrane have been previously demonstrated as a useful biomaterial for tissue engineering Here three laminin active peptides, A99 (AGTFAIRGDNPOG) AG73 (RKRLQVQLSIRT) and EF1zz (ATLQLQEGRLHFXFDLGKGR, X Nle), which interact with integrin alpha v beta 3, syndecans, and integrin 1213, respectively, were conjugated to alginate and evaluated the biological activities A99-alginate (3-3000 ng/min(2)) promoted cell attachment depending on the amount of alginate More than 300 ng/mm(2) of the, A99-alginate matrices effectively promoted cell attachment, cell spreading with well-organized actin stress fibers, and neurite outgrowth AG73- and EF1zz-alginates promoted strong cell attachment at the all amounts (3-3000 ng/mm(2)) A99-alginate (30-3000 ng/mm(2)) promoted strong neurite outgrowth but lower amounts of A99-alginate (3 ng/mm(2)) showed weak activity In contrast, AG73-alginates (3-30 ng/mm(2)) showed strong neurite outgrowth activity but higher amounts of AG73-alginate (300-3000 ng/mm(2)) decreased the activity These data indicate that neurite outgrowth activity of peptide-alginate matrices is peptide specific and the activity is dependent on the amount of alginate Further, biological activities of the peptides on alginate and chitosan matrices were different, suggesting that the integrin- and syndecan-mediated cellular functions on the peptide-matrices are highly influenced by the scaffold structure including polysaccharide types and amounts The laminin active peptide-conjugated alginate and chitosan matrices can control receptor type specific junctions and are useful for tissue engineering (C) 2010 Wiley Periodicals, Inc Biopolymers (Pept Sci) 94 711-720,2010