Journal
MOLECULES
Volume 23, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/molecules23102498
Keywords
Melastoma sanguineum; phenolic compounds; microwave-assisted extraction; response surface methodology; green extraction
Funding
- National Natural Science Foundation of China [81372976]
- Key Project of Guangdong Provincial Science and Technology Program [2014B020205002]
- Hundred-Talents Scheme of Sun Yat-Sen University
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A microwave-assisted extraction (MAE) technology optimized by response surface methodology (RSM) was established to extract phenolic compounds from the fruit of Melastoma sanguineum. The effects of solvent composition, ratio of solvent to material, temperature, time and microwave power on phenol yield were evaluated in single-factor tests. The three parameters exerting main impacts on phenol yield were further optimized by RSM. Under optimal extraction conditions (31.33% ethanol, solvent/material ratio of 32.21 mL/g, 52.24 degrees C, 45 min and 500 W), the total phenolic content was 39.02 +/- 0.73 mg gallic acid equivalent (GAE)/g dry weight (DW). This MAE method performed better in comparison with two conventional methods, those being maceration (25.79 +/- 1.03 mg GAE/g DW) and Soxhlet extraction (18.40 +/- 1.34 mg GAE/g DW), using lower process temperature, shorter irradiation time, and lower organic solvent consumption. In addition, five flavonoids (epicatechin gallate, epicatechin, rutin, pigallocatechin and quercetin) and two phenolic acids (protocatechuic acid and chlorogenic acid) in the extract were identified and quantified using UPLC-MS/MS.
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