Journal
BIOPHYSICAL JOURNAL
Volume 106, Issue 1, Pages L01-L03Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2013.11.1119
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- Deutsche Forschungsgemeinschaft Center for Nanoscale Microscopy and Molecular Physiology of the Brain
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We demonstrate superresolution fluorescence microscopy (nanoscopy) of protein distributions in a mammalian brain in vivo. Stimulated emission depletion microscopy reveals the morphology of the filamentous actin in dendritic spines down to 40 mu m in the molecular layer of the visual cortex of an anesthetized mouse. Consecutive recordings at 43-70 nm resolution reveal dynamical changes in spine morphology.
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