Journal
BIOPHYSICAL JOURNAL
Volume 104, Issue 9, Pages 1886-1892Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2013.03.046
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Funding
- National Foundation for Scientific Research (FNRS)
- Universite catholique de Louvain (Fonds Speciaux de Recherche)
- Region Wallonne
- Federal Office for Scientific, Technical and Cultural Affairs (Interuniversity Poles of Attraction Programme)
- Research Department of the Communaute francaise de Belgique (Concerted Research Action)
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Single-cell force spectroscopy is a powerful atomic force microscopy modality in which a single living cell is attached to the atomic force microscopy cantilever to quantify the forces that drive cell-cell and cell-substrate interactions. Although various single-cell force spectroscopy protocols are well established for animal cells, application of the method to individual bacterial cells remains challenging, mainly owing to the lack of appropriate methods for the controlled attachment of single live cells on cantilevers. We present a nondestructive protocol for single-bacterial cell force spectroscopy, which combines the use of colloidal probe cantilevers and of a bioinspired polydopamine wet adhesive. Living cells from the probiotic species Lactobacillus plantarum are picked up with a polydopamine-coated colloidal probe, enabling us to quantify the adhesion forces between single bacteria and biotic (lectin monolayer) or abiotic (hydrophobic monolayer) surfaces. These minimally invasive single-cell experiments provide novel, to our knowledge, insight into the specific and nonspecific forces driving the adhesion of L. plantarum, and represent a generic platform for studying the molecular mechanisms of cell adhesion in probiotic and pathogenic bacteria.
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