Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 407, Issue 8, Pages 2337-2342Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-015-8532-6
Keywords
MALDI; Transmission geometry; High spatial resolution; MS imaging; Protein imaging
Funding
- National Institutes of Health National Institute of General Medical Sciences NIH/NIGMS [P41 GM103391-04]
- NIH/NIGMS [R01 GM058008-15]
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We have achieved protein imaging mass spectrometry capabilities at sub-cellular spatial resolution and at high acquisition speed by integrating a transmission geometry ion source with time of flight mass spectrometry. The transmission geometry principle allowed us to achieve a 1-mu m laser spot diameter on target. A minimal raster step size of the instrument was 2.5 mu m. Use of 2,5-dihydroxyacetophenone robotically sprayed on top of a tissue sample as a matrix together with additional sample preparation steps resulted in single pixel mass spectra from mouse cerebellum tissue sections having more than 20 peaks in a range 3-22 kDa. Mass spectrometry images were acquired in a standard step raster microprobe mode at 5 pixels/s and in a continuous raster mode at 40 pixels/s.
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