Journal
BIOPHYSICAL JOURNAL
Volume 105, Issue 2, Pages L05-L7Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2013.05.063
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Funding
- Australian Research Council
- National Health and Medical Research Council of Australia
- Human Frontier Science Program
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Identifying the three-dimensional molecular organization of subcellular organelles in intact cells has been challenging to date. Here we present an analysis approach for three-dimensional localization microscopy that can not only identify subcellular objects below the diffraction limit but also quantify their shape and volume. This approach is particularly useful to map the topography of the plasma membrane and measure protein distribution within an undulating membrane.
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