Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 7, Issue 23, Pages 12422-12429Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am507591u
Keywords
upconversion nanoparticles; graphene oxide; FRET; DNA biosensor
Funding
- University of Southampton
- EPSRC [EP/J016918/1]
- Spanish Ministry of Science [MAT2010-15349]
- Juan Palomo Foundation [YMGYMC-01-2014]
- Spanish Ministry of Education [AP2010-1163]
- BBSRC
- EU COST action [CM1101]
- Engineering and Physical Sciences Research Council [1371608, EP/K029150/1, EP/J016918/1] Funding Source: researchfish
- EPSRC [EP/J016918/1, EP/K029150/1] Funding Source: UKRI
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In this work we demonstrate a DNA biosensor based on fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er nanoparticles and graphene oxide (GO). Monodisperse NaYF4:Yb,Er nanoparticles with a mean diameter of 29.1 +/- 2.2 nm were synthesized and coated with a SiO2 shell of 11 nm, which allowed the attachment of single strands of DNA. When these DNA-functionalized NaYF4:Yb,Er@SiO2 nanoparticles were in the proximity of the GO surface, the pi-pi stacking interaction between the nucleobases of the DNA and the sp(2) carbons of the GO induced a FRET fluorescence quenching due to the overlap of the fluorescence emission of the NaYF4:Yb,Er@SiO2 and the absorption spectrum of GO. By contrast, in the presence of the complementary DNA strands, the hybridization leads to double-stranded DNA that does not interact with the GO surface, and thus the NaYF4:Yb,Er@SiO2 nanoparticles remain unquenched and fluorescent. The high sensitivity and specificity of this sensor introduces a new method for the detection of DNA with a detection limit of 5 pM.
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