Journal
BIOPHYSICAL JOURNAL
Volume 102, Issue 6, Pages L23-L25Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2012.01.054
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Funding
- National Institutes of Health [GM087253, GM080376, GM097889]
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Recordings from single molecule experiments can be aggregated to determine average kinetic properties of the system under observation. The kinetics after a synchronized reaction step can be interpreted using all of the standard tools developed for ensemble perturbation experiments. The kinetics leading up to a synchronized event, determined by the lifetimes of the preceding states; however, are not as obvious if the reaction has reversible steps or branches. Here we describe a general procedure for dealing with these situations.
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