Journal
BIOPHYSICAL JOURNAL
Volume 101, Issue 10, Pages L44-L46Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2011.10.019
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Funding
- Ministry of Education, Culture, Sports, Science and Technology
- Grants-in-Aid for Scientific Research [22121521] Funding Source: KAKEN
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Structural diversity of N-glycans is essential for specific binding to their receptor proteins. To gain insights into structural and dynamic aspects in atomic detail not normally accessible by experiment, we here perform extensive molecular-dynamics simulations of N-glycans in solution using the replica-exchange method. The simulations show that five distinct conformers exist in solution for the N-glycans with and without bisecting GlcNAc. Importantly, the population sizes of three of the conformers are drastically reduced upon the introduction of bisecting GlcNAc. This is caused by a local hydrogen-bond rearrangement proximal to the bisecting GlcNAc. These simulations show that an N-glycan modification like the bisecting GlcNAc selects a certain key (or group of keys) within the framework of the bunch of keys mechanism. Hence, the range of specific glycan-protein interactions and affinity changes need to be understood in terms of the structural diversity of glycans and the alteration of conformational equilibria by core modification.
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