Journal
BIOPHYSICAL JOURNAL
Volume 100, Issue 3, Pages L15-L17Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2010.12.3703
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Funding
- National Institutes of Health [RO1GM076665, P41-RR03155]
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Dynamin 2 is an ubiquitously expressed similar to 100 kDa GTPase involved in receptor-mediated endocytosis, Golgi budding, and cytoskeletal reorganization. Dynamin molecules assemble around the necks of budding vesicles and constrict membranes in a GTP-dependent process, resulting in vesicle release. The oligomerization state of dynamin 2 in the membrane is still controversial. We investigated dynamin 2 within the plasma membrane of live cells using total internal reflection microscopy coupled with number and brightness analysis. Our results demonstrate that dynamin 2 is primarily tetrameric throughout the entire cell membrane, aside from punctate structures that may correspond to regions of membrane vesiculation.
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