Journal
BIOPHYSICAL JOURNAL
Volume 100, Issue 7, Pages 1810-1818Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2010.12.3746
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Funding
- Human Frontiers Science Program [RGP66/2004]
- Ministry of Economic Affairs
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek [FOM-L1707M]
- European Research Council Advanced Investigator
- SystemsX
- National Centers of Competence in Research (Chemical Biology. Swiss National Science Foundation)
- R'Equip (Swiss National Science Foundation)
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A multitude of biological processes that involve multiple interaction partners are observed by two-color microscopy. Here we describe an analysis method for the robust quantification of correlation between signals in different color channels: particle image cross-correlation spectroscopy (PICCS). The method, which exploits the superior positional accuracy obtained in single-object and single-molecule microscopy, can extract the correlation fraction and length scale. We applied PICCS to correlation measurements in living tissues. The morphogen Decapentaplegic (Dpp) was imaged in wing imaginal disks of fruit fly larvae and we quantified what fraction of early endosomes contained Dpp.
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