Albacore tuna spleen trypsin: Potential application as laundry detergent additive and in carotenoprotein extraction from Pacific white shrimp shells

Partitioned trypsin from spleen of albacore tuna (Thunnus alalunga) by ATPS was evaluated for its potential applications in laundry detergents and in the recovery of carotenoprotein from Pacific white shrimp (Litopenaeus vannamei) shells. The partitioned trypsin was extremely stable toward various surfactants and bleach agents and showed excellent stability and compatibility with commercial liquid and solid detergents. Additionally, partitioned trypsin showed an efficient hydrolysis and recovery of carotenoprotein from Pacific white shrimp shells. The carotenoprotein recovery was maximized by the hydrolysis of shrimp shells using 0.8 trypsin units/g shrimp shells at 25 degrees C for 45 min and shrimp shells/buffer ratio of 1: 2 (w/v). Carotenoprotein consisted of 72.4% protein, 18.8% lipid, 7.1% ash, 1.6% chitin, and 73.3 mu g total astaxanthin/g sample. It was rich in essential amino acids. When the hydrolytic activities of albacore tuna and bovine trypsins used in the extraction of carotenoprotein in Pacific white shrimp shells were compared, the recovery efficacy of protein and pigment by albacore tuna trypsin was similar to that achieved by bovine trypsin. These results suggest that albacore tuna trypsin could be used as an ideal choice for application in detergent formulations and for extraction of carotenoproteins.