Journal
BIOPHYSICAL JOURNAL
Volume 98, Issue 4, Pages 543-551Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2009.11.001
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Funding
- Landesstiftung Baden-Wurttemberg
- European Umon-STREP NanoCues
- Alfried-Krupp von Bohlen und Halbach-Stiftung
- Max-Planck-Society At Heidelberg
- Center for Modelling and Simulation in the Biosciences (BIOMS)
- Cluster of Excellence CellNetworks
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Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cell adhesion For spacings >= 90 nm, focal contact formation was inhibited and the detachment forces as well as the stiffness of the cell body were significantly decreased compared to spacings <= 50 nm Analyzing cell detachment at the subcellular level revealed that rupture forces of focal contacts increase with loading rate as predicted by a theoretical model for adhesion clusters Furthermore, we show that the weak link between the intra- and extracellular space is at the intracellular side of a focal contact. Our results show that cells can amplify small differences in adhesive cues to large differences in cell adhesion strength
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