Journal
BIOPHYSICAL JOURNAL
Volume 98, Issue 9, Pages 1856-1863Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2009.12.4333
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Funding
- European Commission
- National Human Genome Research Institute, National Institutes of Health
- Royal Thai Government
- Thailand National Science and Technology Development Agency
- Merage Foundation
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The staphylococcal a-hemolysin (alpha HL) protein nanopore is under investigation as a fast, cheap detector for nucleic acid analysis and sequencing. Although discrimination of all four bases of DNA by the alpha HL pore has been demonstrated, analysis of single-stranded DNAs and RNAs containing secondary structure mediated by basepairing is prevented because these nucleic acids cannot be translocated through the pore. Here, we show that a structured 95-nucleotide single-stranded DNA and its RNA equivalent are translocated through the alpha HL pore in the presence of 4 M urea, a concentration that denatures the secondary structure of the polynucleotides. The alpha HL pore is functional even in 7 M urea, and therefore it is easily stable enough for analyses of challenging DNA and RNA species.
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