Journal
BIOPHYSICAL JOURNAL
Volume 98, Issue 1, Pages 164-173Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2009.09.047
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Funding
- University of Houston, Rice University
- Robert A. Welch Foundation [F-1514]
- National Science Foundation [CHE 0347862, CHE 0848571]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0848571] Funding Source: National Science Foundation
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A method to denoise single-molecule fluorescence resonance energy (smFRET) trajectories using wavelet detail thresholding and Bayesian inference is presented. Bayesian methods are developed to identify fluorophore photoblinks in the time trajectories. Simulated data are used to quantify the improvement in static and dynamic data analysis. Application of the method to experimental smFRET data shows that it distinguishes photoblinks from large shifts in smFRET efficiency while maintaining the important advantage of an unbiased approach. Known sources of experimental noise are examined and quantified as a means to remove their contributions via soft thresholding of wavelet coefficients. A wavelet decomposition algorithm is described, and thresholds are produced through the knowledge of noise parameters in the discrete-time photon signals. Reconstruction of the signals from thresholded coefficients produces signals that contain noise arising only from unquantifiable parameters. The method is applied to simulated and observed smFRET data, and it is found that the denoised data retain their underlying dynamic properties, but with increased resolution.
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