Journal
PANCREAS
Volume 48, Issue 5, Pages 711-718Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/MPA.0000000000001310
Keywords
recurrent acute pancreatitis; acinar cells; apigenin; parathyroid hormone-related protein
Categories
Funding
- NIH [P01 DK035608, K08 CA125209, T32 DK7639]
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [T32DK007639] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Objective: Chronic pancreatitis is the consequence of multiple episodes of recurrent acute pancreatitis (RAP). We hypothesized that apigenin can minimize the sequelae of RAP by limiting acinar cells' proinflammatory signaling pathways. Methods: AR42J acinar cells were treated in vitro with transforming growth factor beta (TGF-beta), apigenin, and other inhibitors. Dual luciferase reporter assay measured parathyroid hormone-related protein (PTHrP) promoter activity. MAPK/ERK pathway activity was assessed by immunoblotting and in vivo by immunohistochemistry with a cerulein-induced RAP mouse model. Nuclear factor kappa B nuclear localization was analyzed in vitro in cells stimulated with tumor necrosis factor alpha. Primary acini were isolated and treated with cerulein; interleukin 6 messenger RNA was measured comparing PTHrP wild-type and knockout mice. Results: Apigenin and PD98059 each downregulated TGF-beta stimulation of PTHrP P3 promoter activity. In a RAP mouse model, apigenin reduced pERK nuclear localization in acinar cells and preserved acinar cell architecture. Apigenin suppressed tumor necrosis factor alpha-mediated signaling by decreasing nuclear factor kappa B nuclear localization and decreased interleukin 6 messenger RNA levels via a PTHrP-dependent mechanism. Conclusions: Apigenin reduced inflammatory responses in experimental models of RAP. The mechanisms mediating the actions of apigenin, in part, are owing to attenuation of PTHrP and TGF-beta proinflammatory signaling.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available